Exploring the anti-fatigue properties of ginseng stem and leaf saponins: Using UHPLC-MS metabolomics and neurotransmitter analysis 2025 Wu et al

*in exhausted mice

Highlights

• The anti-chronic fatigue syndrome mechanism of ginseng stem-leaf saponins was analyzed in terms of both peripheral and central fatigue.
• Fourteen neurotransmitters were analyzed simultaneously, including monoamines, amino acids, and cholines.
• Potential mechanisms of ginseng stem-leaf saponins for the treatment of chronic fatigue syndrome were analyzed using metabolomics.

Abstract

Background
Chronic fatigue syndrome (CFS) is a crippling illness with limited effective treatment options. Recent findings have suggested that ginseng stem and leaf saponins (GSLS) possess anti-fatigue effects. However, the detailed mechanisms by which GSLS impact CFS have yet to be fully elucidated.

Purpose
This study aimed to explore the efficacy and underlying mechanisms of GSLS in the treatment of peripheral and central fatigue in mice with CFS.

Methods
A CFS model was established in male C57BL/6 mice, which were then subjected to prolonged swimming tests. The validity of this model and the efficacy of GSLS were verified using the forced exhaustion swimming test and the Morris water maze test. The anti-fatigue mechanism of GSLS was verified using pathological section observations, biochemical index tests, neurotransmitter analysis, and Ultra-high performance liquid chromatography–high-resolution mass spectrometry (UHPLC-MS) metabolomics analysis.

Results
GSLS prolonged the swim time-to-exhaustion in CFS mice and reduced escape latency in the water maze, revealing their anti-fatigue and memory-improving effects. GSLS significantly protected both peripheral (liver) and central (hippocampus) tissues, as shown in pathological sections. Biochemical indices suggested that GSLS mitigate peripheral fatigue by reducing energy expenditure and oxidative damage and inhibiting the production of harmful metabolites. Furthermore, GSLS altered the abnormal levels of 14 neurotransmitters in the brains of CFS mice, including seven upregulated and seven downregulated neurotransmitters, highlighting their role in mitigating central fatigue through neurotransmitter regulation. Serum metabolomics analysis identified alterations in 19 metabolites across 11 metabolic pathways, thereby elucidating the potential mechanisms by which GSLS treat CFS.

Conclusion
GSLS ameliorate damage caused by both peripheral and central fatigue. They also act on multiple pathogenic mechanisms by modulating neurotransmitters and serum biomarkers, making them a potential therapeutic agent for CFS.

Paywall

 

To quote the immortal John McEnroe:
"Are you serious?"

 

Do the authors think you can acquire ME/CFS by swimming too much?

 

Apparently, yes.

Chronic fatigue syndrome (CFS) is a debilitating, long-term illness characterized by profound fatigue that significantly impairs daily functioning. Initially presenting as generalized weakness and lethargy, CFS may progress to a severe reduction in motivation and interest with potential psychological consequences, including major depressive disorder (Sandler and Lloyd, 2020).

https://www.s4me.info/threads/chron...d-possibilities-2020-sandler-and-lloyd.14510/

 

Issues with swimming? Must be a problem of too much strudel.