@wigglethemouse In your table from Biobank, there is a column labeled HWE, which I imagine is an abbreviation for Hardy-Weinberg equilibrium. Can...
Chris, Does "Variant annotation dubious" mean that the Broad investigators think the existence of the variant is dubious or do they mean that...
I used this phrase because the strategy we employed was to search for genes coding for enzymes or transporters with common damaging mutations....
@Chris Ponting I'm always going to defer to your expertise on genomics. But I am going to ask questions. First question is this. Some people tell...
Yes. I suggested all of those as reviewers. Also Travis Craddock in the Broderick group.
This question can be seen as a variant of your sudden vs. progressive onset question. Trapping more cells of a given cell type or trapping new...
An upper limit on cellular Trp? Certainly there is the limit of solubility, but below that the level achieved in any cell will depend on the...
To answer @Robert1973, your post includes many questions. I'll do my best. The IDO metabolic trap hypothesis does predict a stable steady state...
Thanks to @Michiel Tack, I now have a PDF of the Streeten (Bell) (2000) paper on erythrocyte volume in ME/CFS. Table 4 of that paper shows that...
This topic has interested me so, like Michiel, I've tracked down some of the published work. I think Michiel is correct that David Bell often...
As Chris points out there are many SNVs in IDO2. The IDO metabolic trap hypothesis, however, is not based on a GWAS approach to the data. The...
This is an interesting and important question. I'll look into it. I will, however, be greatly surprised if all our CFS patients (now n=66)...
There is a video of a talk by Michael Maes on the Australian ME group's (eMErge) site. Early on in the talk, he quotes two European government...
I'm assuming your "below 15% of the RDA" means below 15% of the normal reference range for plasma vit B3. You're right there could be a limitation...
Good critical question. When I first encountered the 5 mutations in IDO2, I was looking at genes one by one, manually. I started with all the...
We are already measuring cell serotonin in our mixed population of PBMCs, but it's likely that only the dendritic cells are in the trap, so it may...
Yes, depending on which isoform of TPH (the first enzyme in the Trp-to-serotonin pathway) is expressed in serotonergic neurons. One isoform is,...
The IDO metabolic trap cannot occur in any cell type where TDO2 is is expressed. The TDO2 gene product does not undergo substrate inhibition, so...
Good question. For the moment, we are relying on the high probability that any normal control has a 40% chance of being at least heterozygous for...
If you have seen single cell RNAseq data for cell types where only IDO1 is expressed and not IDO2 or TDO, please point them out to me. One place...
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