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I was basing that on something that Tony Komaroff had said/written (sorry, can’t remember where). That would tie in with his 1991 research you mentioned, presumably it was that that fed into the 92 definition. Though the fact, they use the term postexertional malaise in the abstract without...

Have you thought of contacting Maureen Hansen to ask if she’s done or considered the studies you suggest? I think you would get a response.

Good to hear that it might not be surprising not to see a signal of CD8 exhaustion in the single cell RNA data they already have. it’s great to have these insights from the lab.

With pretty much all hypotheses, a GWAS study is a good place to look for confirmatory data. The unknown is whether or not DecodeME will be big enough to pick up most signals. Firstly, until you've done a GWAS, you won't know how strong the genetic signal is (unless you already have big robust...

RE expecting to see signs of CD8 T-cell exhaustion in the RNA data: Thanks. This 2019 review says similar CD8+ T cell exhaustion Exhausted T cells are characterized by progressive loss of effector functions (cytokine production and killing function), expression of multiple inhibitory...

This is certainly interesting work, particularly the new, unpublished findings about the “exhausted CD8 T cells signature” of PD-1 and transcription factors. And I like the way that it builds from the early metabolic work into the flow cytometry stuff and the link into the single-cell RNA work...

it was, but it seems to be very accurate. In other words, it’s not clear that this somatic symptoms are particularly helpful in diagnosing depression, while they drag in lots of non-depressed people with chronic illnesses. ultimately, you need a clinician to diagnose depression, and I’m not...

I did look into this at one point. Try the PHQ-2 https://www.hiv.uw.edu/page/mental-health-screening/phq-2 This drops all the somatic symptoms, and instead focuses on two simple qualities: – feeling low. – lack of pleasure There is some published research that compares the PHQ-two with the...

Thanks for such a helpful explanation. I agree that the concern is they use a black box method. I think the idea with decodeme is to take a sample, randomly split it into two cohorts, and then see if they can replicate the results from the test cohort in the replication one. I think that...

Because it’s a combinatorial approach, rather than the traditional GWAS, single – SNP approach, yes, this is a meaningful cohort. I haven’t read the paper and I’m not sure how clear cut the findings are. I’d like to see the results replicated/validated in independent cohorts. They say they’re...

@chillier thanks for the very helpful analysis and explanation (and for confirming that it is the same salt concentration as used in Ron Davis' experiment). It looks interesting, but they don’t replicate the nanoneedle findings. However, this experiment seems to have very significant...

My mistake - I meant shrink (I'm going to blame my migraine for that one!) Except OMF are in the happy situation of having lots of funds. They say they are applying for NIH funds as Esfandyarpour's condition to work on the project. The money will go to him. So I don't see the benefit to OMF...

One more question to address: why hasn't OMF pursued impedance testing? 1. The nanoneedle is cutting edge and proprietary technology, and unfortunately its inventor has no particular interest in ME. He left Stanford, and is apparently now leads his own research group at University of California...

Are you saying the cells were already dead at the start of the experiment? Or that they might have been killed by hyperosmotic stress without first enlarging (@chillier or @Creekside said a volume change is normal in cells that are stressed by hyperosmotic conditions)? I assume the authors...

I am still confused. Are these molecules in the plasma of patients or controls, or molecules introduced by the experimental process as an artefact? If the former, that's still interesting biologically. If an artefact that could account for the case/control differences, I'm not sure how that...

There are a couple of reasons there might be better explanations. 1. I'm not sure how much research was actually done after the work for this research. Throughout, the researchers were hampered by the slow speed of their setup, and manufacturing issues. Hence the work to develop a better system...

If the data is that good, will they be publishing it? I guess the lack of specificity is not necessarily what the ME community wants to hear, but been able to distinguish between sick and healthy. It would be quite something.

Interesting. Can you say any more - e.g. when is the info from and based on how many subjects? Yes, the nanoneedle was developed to be used with a range of sensors that allowed them to detect very specific molecules. The use here seems to have been off-piste. So it's plausible that this level...

Missed that- what kind of difference though? This is an important point. It looks like a mixed picture - but note that 5/5 seem to have been processed in sync, so I don't think that could explain the result (all the controls look much the same, however the samples were collected). Sample...

Thanks for all the comments, though I'm afraid the discussions of the nanoneedle itself are over my head. My working assumption is that whatever changes the nanoneedle detected, they are likely to be significant given the total separation between cases and controls. Plus, I think this is the...