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I wouldn't relate these observations to body-wide issues or dietary aspects. This observation is most likely to be a consequence of a cell type specific effect, and even if it wasn't, we haven't got evidence of that yet.

So yeah this is why in the last response to Hutan I'd mentioned lipids that clearly stood out as opposed to the whole dataset for the PCA - it was clear that the top end, particularly this one, were behaving far more dramatically different than the rest, so this behaviour formed part of the...

It was already in the paper before that other thread existed. So, I thought, okay, this debate is extending beyond my confident knowledge of statistics and I don't want to do something wrong, so let's consult a colleague (an Accredited Statistician of the Statistical Society of Australia) who we...

The choice was made prior to obtaining any data, I decided to be thorough and include the whole pipeline so as not to keep any results from the community. That includes negative results. Correct, and none. It is based on the levels of that metabolite within context of levels of each metabolite...

I've just gotten back to the office so I haven't read the whole thread yet but just saw this comment (edit: I am editing in more responses as i see them). I'm not sure why it's raising a red flag for shoehorning so I'll explain what I did. I put the features into metaboanalyst and those three...

We're introducing some techniques to our lab that will let us do varied metabolic assays on specific immune subsets in high throughput. In terms of single cell, maybe, it would be good to complement what we are intending to do. But that would be dependent on the budget of whatever grants we can...

Yeah that's the next step. I would not make this claim using this data.

Thanks!!! I need to pick our conversation back up as well. In truth I have been unable to work for some weeks due to vertigo so I've dropped the ball on some things over the new year :( oh and PS: this whole membrane and BCR thing is my personal interpretation of what the next most useful...

I was surprised to get an email saying that this was out prior to final publication (which is delayed only due to administrative delays caused by the festive season)... I don't recall approving something like this (I don't like pre-prints, I feel it muddies the waters when there are two versions...

Gotcha. Maybe I jumped in without it.

I understand and agree with where this is coming from but I think this is an uncharacteristically dangerous sentiment to promote bluntly. Stuff should really be dismissed based on the details, not on first impression based on whether it was missed or not (or some other aspect not necessarily...

We'll never stop biobabble with words. It's like trying to stop the tides. I have driven myself grey and to sleeplessness and stomach pain trying to do so. Memes are too strong. Even as a mitochondrial expert, probably the longest in the tooth for continuous wet lab activity in ME/CFS research...

Many papers published this year on mitochondrial function in PBMCs (particularly Seahorse one in Long COVID) and we will continue to get clinical trials of mitochondrial "enhancers" (as if anything in biology is so straightforward that taking any drug or supplement is implicitly a "good" thing)...

Stomach ache inducingly so.

Have to wait for the paper to be out for the smarter forum members to scrutinise results (To be clear, I mean smarter than me)

I have no idea what is typical when using this technique to test samples from people (whether this is a thing that anybody else does in other disease contexts, I also don’t know)

I'm not surprised contradictions are making it through peer review. One of my recent submissions (to a good, non-predatory journal no less) had 3/4 reviews that were very obviously entirely assembled by LLMs.... it's become a total farce.

Just to address the question of the method, patch clamping is notoriously difficult

I attended days and days' worth of talks about B cells the week before last. Phenotypes change so much with the most subtle of context changes, even within different regions of the same GC. I can't imagine how one would combat spatial sampling artefacts if aspirating by fine needle from lymph...

I gave 100 samples to a collaborating T cell lab a week or two ago, will have some interesting results to follow this up with maybe next year