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Looks interesting, will be good to follow what Wuest and his team do. I don't know much about how to interpret muscle histological data like this, but - like with the WASF3 paper - I'm concerned about what the effect of deconditioning will have on muscles and therefore these results. Would be...

I don't remember seeing proteomics done on CSF before, looking forward to seeing the results in full.

I'm a bit confused, what is the "self interest of charities" in reference to? EDIT: Just seen you mean the embedded tweet that Stuart Murdoch is replying to - yes, I of course agree that it's a ridiculous claim

To clarify some of the less clear activity labels from the bar plot above: physical activity is used to describe things likes brushing teeth, taking a bath, making food and so on. Petting dog should probably have been labelled with physical activity. problem solving usually means things like...

Metadata Here are some general properties of the data I've collected. As a reminder, a data point is a 10 minute block of time where I have recorded roughly what I was doing and may have features such as saccades and key presses etc associated with it. In my activity log I have recorded 3068...

Just saw this tweet from the famous musician and ME sufferer Stuart Murdoch. His twitter account @nee_massey has 36.5K followers and his band account @bellesglasgow 98.6K followers. I searched his twitter history and haven't seen any mention of DecodeME (although it's possible he may have...

I've done the densitometry myself and quantified relative to GAPDH as they would presumably have done (a "housekeeping" gene there to normalise for total protein levels). Bear in mind I'm working with the snippets of the blot membranes they are showing in the paper and not the membrane itself...

haha thankyou, it is. They're beautiful aren't they. There's also something poetic about how their land dwelling relatives might be the ugliest animals on the planet.

Interesting, my fitness band also measures stress but I've never really looked at it. Looking at it now it seems like it's been taking measurements for me too infrequently to be able to marry up to the data I collected here. In my case I've no idea!

This is really interesting thankyou it sounds like you're really familiar with this technique. Agree about passage number in particular and didn't know about contact inhibition that's interesting. Out of interest is there data showing differences in seahorse results with changes in confluence...

This figure is the bit that really matters for sure. They have taken muscle biopsies from these 10 controls and 14 ME/CFS patients (from the intramural study i think so should be diagnosed well) and done western blots on them for WASF3, MTCO1, COX17, PERK and BiP. Here's the rest of the figure...

Good idea! although I think I'm looking at this from a different angle. Here I'm really trying to find good ways to track how much cognitive work a person doing, in the same way a wrist band can measure steps as a proxy for physical activity. So then it could be used as as an aid to check if you...

Too long, didn't read: I've stopped collecting data and plan to analyze it. If the data is useful then the differences should be obvious but I've 'pre-registered' a mini analysis here anyway. This is not necessarily the best plan and I'm very open to critique. Most analysis will be exploratory...

From the research perspective I think there's a distinction to be made between clinical research and basic research in the lab. One of the biggest difficulties from the wetlab perspective is that getting hold of samples is insanely hard. In terms of biobank there's really only CureME and that's...

Listened to most of it - agree with what people have said here. I think when it comes to e-training materials more than anything it needs to be made clear to doctors that exercise is dangerous - I don't think that point has been made firmly enough. That and any sort of superstition than you can...

Ah fantastic! Here's the link for non twitter users: ow.ly/fvmj50PvPPT

It's the kind of thing that would be good to have an audio version of or maybe a digest so it's accessible to as many pwME as possible

Thank god - untampered with I trust!

Sonya chowdhury of action for ME says: " I’m delighted that the cross government national #MECFS interim delivery plan will be launched for consultation later today. @actionforme Thanks to @thetimes for their coverage this morning raising much-needed awareness"

Interesting why is that? I'm familiar with in situ hybridisation from developmental biology where it is a routine way of looking at expression domains. Why is it a problem with viruses? low copy number or maybe the RNA being bound up in viral assembly proteins? In the lab I formerly worked in we...