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For me the shakiest part of their argument is suggesting that PDH inhibition is the cause and that the increased respiratory capacity they see in their seahorse data (the part Audrey and I are attempting to replicate) represents an adaptation. It's not clear that the seahorse data represents the...

I'm not sure, but isn't the question of regulation of amino acid use also linked to the question of why pyruvate dehydrogenase is inhibited in the first place. The whole regulatory situation is a mystery isn't it? In general it seems perfectly plausible that the cell would compensate for a lack...

Tronstadt's group do another metabolomics study in a 2021 paper, and don't really replicate their 2016 result in their whole ME cohort but do argue that they see some branched chain amino acid derivates indicative of amino acid's being used for energy. They cluster the ME patients into three...

The muscle metabolomics done by Rob Wust's team in long covid would fit fairly well with that: If glutamate and alpha-ketoglutarate are feeding the TCA then their levels will be low, it'll travel around the remaining 3/4s of the TCA cycle (where most of the energy generating steps are), but...

I'm quite pleased to see these changes. I would still prefer if the link text itself referred to mecfs rather than cfs, but the most important thing is the addition of PEM.

Moved posts I think there was a thread about this but I can't find it so posting here. I think the NHS website for ME/CFS has been improved. https://www.nhs.uk/conditions/chronic-fatigue-syndrome-cfs/ - PEM listed as a main symptom - GET mentioned as unsuitable Is this old news that I have...

The amino acid results in Tronstadt's 2016 paper seem to be pretty much a female only effect. Interesting! I don't know unfortunately, maybe it'll be good to read up on that.

So true! I can't imagine what would happen to me if I did a CPET. Must be dangerous for so many people. Maybe something like longitudinal sampling combined with an activity tracker? Collecting regular urine samples would be easy at least.

Good point about group 3. Group 2 generates acetyl CoA but group 3 don't so you would expect levels of oxaloacetate/ malate to increase and citrate levels to be low. You would still get a lot of the electron transport chain substrates generated from the krebs cycle post alpha ketoglutarate...

My concern with the amino acid findings in Tronstad 2016 is that it doesn't seem to replicate very well. Amino acid differences are not really found in two of lipkin's metabolomics studies, either of two metabolomics studies by Hanson's group (at baseline), Naviaux's metabolomics study (some...

This thread's paper also relies heavily on Gene Ontology (GO) term enrichment analysis, which Grimson et al also do. Gene Ontology terms are short phrases that describe a gene's function, cellular location and so on. For example a cytoplasmic gene involved in cell signalling may have...

The Grimson paper is much better powered (n~30 per group I believe compared to n~4 here). The two papers disagree in the abundances of immune cells types. This threads paper reports significantly decreased monocyte and NK cell abundances and increased T cell abundance. Grimson et al report...

I believe it was a 2 year gap though that's long enough that the actual relevant series of events could be different to how I perceive them to be. - 2010 'infection' with symptoms of intense fatigue and some neurological symptom where my fine motor movements were sluggish, lasted 3 months then...

In Armstrong et al 2015's metabolomics study (n=34 patients n=25 controls) in ME the only significantly elevated metabolite they see in the blood is glucose (phenylalanine/glutamate/hypoxanthine significantly low): In Germain and Hanson 2022's 2 day CPET metabolomics study 1 of the 7...

Here are some of the pieces of relevant literature that I am aware of: Beentjes and Ponting's recent preprint of blood factors in the UK biobank cohort. Many of the findings in the 'blood biochemistry' section are associated with insulin resistance including high(er) glucose, hba1c, and a high...

I bought a continuous glucose monitor a while ago thinking it could be interesting to try. @TamaraRC 's post has inspired to me to finally put it on. Here's a trace from when I had 5 pieces of a medium pizza yesterday evening (peak on the right): and a relative of mine eating exactly the...

For me it's as simple as this. When I was moderate but still unable to work/study early in my illness I was mostly sitting. When I'm severe lying down is essential. If I'm sitting these days I find I automatically raise my legs up onto something if there's a way to do that. I've had relapses and...

Great clip really well communicated, which is difficult to do. My only suggestion would be that it's not so much it is/was thought to be 'psychological,' as it is that symptoms are 'made up' consciously or unconsciously because pwME are either crazy or lazy and for some kind of secondary gain...

@TamaraRC you have a slide about glucose tolerance testing on over n=750+ by McGregor. The figure you show appears to talk of small sample sizes n < 25. Do you know is this data published? I can only find an article that Cort Johnson wrote about in 2019.

@forestglip was 2-hydroxybutyrate significant in that study? Is it possible to get the mann whitney p value for this? I wasn't clear in your post whether it was significant or simply alphabetically at the top of the list of the all metabolites with a positive fold change.