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Different viruses have different impacts on stress granule formation, some increasing it, some decreasing it. So there's a delay of about 24 hours between the stress on the cell and the up regulation of the viral transcription. (Perhaps that fits with PEM?) I think the authors are suggesting...

There's this study GTPase-activating protein-binding protein 1 (G3BP1) plays an antiviral role against porcine epidemic diarrhea virus, 2019, Pandey et al It's suggesting that reduced G3BP1 reduces stress granule formation and increases the success of a virus in replicating in pigs...

Edit - the RAS GTPase-activating protein 1 and the RAS GTPase-activating protein-binding protein 1 are fact different, despite the very similar names. So, ignore this post. See later post. [Googling to see if anyone has reported on Ras GTPase activating protein-binding protein in ME/CFS, I...

I've made as thread to discuss Ras GTP-ase activating protein-binding protein 1 here: Ras GTP-ase-activating protein-binding protein 1

Some general discussion about calcium signalling has been moved here: Calcium signalling; calcium channels

Ras GTPase activating protein binding protein 1 This molecule was mentioned in a paper - levels of the molecule were used as a measure of cell stress (higher levels correlated with higher cell stress): Molecular mechanisms of stress-induced reactivation in mumps virus condensates, 2023, Xiaojie...

Yes. Two types of chemical stress, and heat stress. The paper has links to detailed descriptions of methods.

Viral replication and release are accelerated by stress I marvel at the techniques. There's a protein (RasGTPase protein-binding protein) that is claimed to be a measure of cellular stress. And fluorescent markers of both viral N protein and viral RNA. They have cells infected with the...

Viral factories Membrane-less inclusions in cells that can be seen with electron microscopy, containing viral genomes and replication machinery. Graphical abstract

German research Thanks for posting @Mij. If I had to pick one theory on the cause of ME/CFS, I think it would probably be something to do with reactivations of pathogens that have been sitting latent in cells. I'm only a few paragraphs into the paper, but I think this is looking to be worth...

I wonder if the slowness to bring the results into the public is because of a desire to get all the ducks in a row, to make sure things are unassailable so that people within the NIH and in politics who don't believe in ME/CFS as a biological illness can't easily attack it? A sort of going slow...

I wondered about that, but Figure 1A plots the individual results for the healthy controls, and they look to be quite tightly clustered.

Walitt's views were pretty bad, and, extraordinarily, enough people with decision-making power in the NIH thought that Edward Shorter's extremely misogynistic views about CFS were interesting enough to have him come to the NIH during the ME/CFS study. So, I think some concern is warranted...

Thanks @CRG! Dotted line is the healthy control mean plus 2 standard deviations. The chart indicates that there is a clear increase in the level of anti-CHRM1 antibodies in about half the CFS participants, but not in healthy controls. It looks pretty nice. "cpm" is counts per minute...

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409949/ Gambierdiscus species are the producers of the marine toxins ciguatoxins and maitotoxins which cause worldwide human intoxications recognized as Ciguatera Fish Poisoning. A deep chemical investigation of a cultured strain of G. belizeanus...

From an information sheet about Ciguatera https://www.whoi.edu/science/B/redtide/illness/ciguatera_fish_poisoning.html One of the toxins present in varying amounts in Ciguatera fish poisoning, that we haven't discussed before is maitotoxin. It increases calcium ion influx through excitable...

A reminder about this call - it will be interesting to hear what the NIH is saying on 'updates on ME/CFS-related research activities'. Note the need to register in advance.

An old study that has been included in a list of studies finding issues with G protein-coupled receptors. https://pubmed.ncbi.nlm.nih.gov/12851722/. Paywall Abstract The disturbance of the central nervous system and immunological abnormalities have been suggested in patients with chronic...

My brain has moved to treacle mode. Yeah, I edited out that sentence about them knowing the concentration of the 7S DNA, as I wasn't sure.

I'm not surprised that you say this, as, the study looked pretty patchy to even me, who doesn't know the technicalities. They may have standardised the amount of DNA applied to each sample of microglia. But yes, there's quite a lot that they have not told us.