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Im confusing things here a little bit. The g stands for gravity not grams and based on a calculation with radius of the centrifuge itself. I will check the machine and adjust accordingly. It might have been close to the correct speed to begin with.

That could absolutely be true. The sample was 2-3ml so I need to try this again at double to triple speed. That could maybe actually also sort out the the clots/debris visible. I will post an update if I manage to do this again and get a control as well.

I believe so. At least it is similar to the formations shown in the paper. In a perfect world I would have to die it and view in a electro microscope.

It is important to note that it is possible that the clots are forming in vitro but that would still be an indication of some sort of activation if controls turn out to be clean.

I think I might have been able to replicate the results on my own blood plasma as you can see on the attached image. There were a couple of red blood cells that escaped centrifugation that act as a size reference. The whole sample was scattered with microclots of this size. I'm working on...

Reisa Petrious is on fire. Already with a preprint of a experimental drug treatment: https://www.s4me.info/threads/combined-triple-treatment-of-fibrin-amyloid-microclots-and-platelet-pathology-in-individuals-with-long-covid-pacs-2021-pretorius-et-al.24029/

Combined triple treatment of fibrin amyloid microclots and platelet pathology in individuals with Long COVID/ Post-Acute Sequelae of COVID-19 (PASC) can resolve their persistent symptoms Etheresia Pretorius, Chantelle Venter, Gert Laubshder, Maritha Kotze, Kelebogile Moremi, Sunday Oladejo...

@Jonathan Edwards this also reminds me on the informative study you once referenced: https://www.s4me.info/threads/immunoglobulin-g-structure-and-rheumatoid-factor-epitopes-2019-maibom-thomsen.12294/ There its shown that slight heat treatment of pure plasma creates sediments in vitro because...

I agree this is something that us elevated at the lab. I'm going to refine my theory that the plasma in ME/CFS and LC patient is full of autorective antigens towards soluable exoproteome (not only cytokines but all possible kinds including GPCR antibodies as shown in the REAP study) and immune...

This was covered on this thread: https://www.s4me.info/threads/reap-a-platform-to-identify-autoantibodies-that-target-the-human-exoproteome-2021-wang-et-al.20747/ But I agree that if the microclots are 100microns across then it is even larger than a cell and my theory might not stand completely...

My theory is: microclots = micro immune complexes During viral infection the B cells of the adaptive immunity produce vast amounts of different antigens that some are autoreactive to various self proteins in the blood. This has been shown by Akikio at Yale. These autoantigens towards small...

Here is a presentation by Aaron M Ring. It explains the concept in details and during QnA he confirms they are looking into both long covid and ME/CFS with this technology. Very promising.

One of the difficulties in studying and treating long COVID is that we don’t currently have a definitive way to diagnose it. And without a diagnostic test, the condition becomes much harder to manage or study. Eva Higginbotham spoke to Danny Altmann, an immunologist from Imperial College London...

It is very interesting looking into NETs (Neutrophil Extracellular Traps) part of this. Seems to play a major role in infections, auto immunity and cancer. A new and promising field worth looking into.

Based on this viewpoint, who will be first in line of us to try depleting all B plasma cells with off label use of daratumumab/darzalex? Were do I sign up?

My C3 and C4 are constantly slightly under lower cut off. Latest C3 was 0,71g/L (lower cut off 0,74g/L) and C4 0,13g/L (lower cut off 0,14g/L). Based on litrature I have read about Lupus this should indicate a complement activation of some sort but no doctor has yet put any meaning into it since...

This paper correlates almost completely with my own conclusions about the parthenogenesis of ME/CFS based on my own disease progression, lab results and general investigations. My only addition is that I believe that there is a complement activation for clearance of soluble immune complexes...

Exploring the genomic dark matter: endogenous retroviruses and immunity Presentation by the lead author

Antibodies against human endogenous retrovirus K102 envelope activate neutrophils in systemic lupus erythematosus Maria Tokuyama, Bronwyn M. Gunn, Arvind Venkataraman, Yong Kong, Insoo Kang, Tasfia Rakib, Michael J. Townsend, Karen H. Costenbader, Galit Alter and Akiko Iwasaki Journal of...

Thanks for sharing the article. Very interesting and in line with my thoughts. There is going to be major developments on this on the coming years.