Curcumin ameliorates CKD-induced mitochondrial dysfunction and oxidative stress through inhibiting GSK-3β activity, 2020, Wang et al

Andy · Jun 13, 2020

Curcumin has been reported to attenuate muscle atrophy. However, the underling mechanism remains unclear. The aim of this study was to investigate whether curcumin could improve chronic kidney disease (CKD)-induced muscle atrophy and mitochondrial dysfunction by inhibiting glycogen synthase kinase-3β (GSK-3β) activity. The sham and CKD mice were fed either a control diet or an identical diet containing 0.04% curcumin for 12 weeks. The C2C12 myotubes were treated with H2O2 in the presence or absence of curcumin. In addition, wild-type and muscle-specific GSK-3β knockout (KO) CKD model mice were made by 5/6 nephrectomy, and the sham was regarded as control.

Curcumin could exert beneficial effects, including weight maintenance and improved muscle function, increased mitochondrial biogenesis, alleviated mitochondrial dysfunction by increasing adenosine triphosphate levels, activities of mitochondrial electron transport chain complexes and basal mitochondrial respiration and suppressing mitochondrial membrane potential. In addition, curcumin modulated redox homeostasis by increasing antioxidant activity and suppressed mitochondrial oxidative stress. Moreover, the protective effects of curcumin had been found to be mediated via inhibiting GSK-3β activity in vitro and in vivo.

Importantly, GSK-3β KO contributed to improved mitochondrial function, attenuated mitochondrial oxidative damage and augmented mitochondrial biogenesis in muscle of CKD. Overall, this study suggested that curcumin alleviated CKD-induced mitochondrial oxidative damage and mitochondrial dysfunction via inhibiting GSK-3β activity in skeletal muscle.
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Open access, https://www.sciencedirect.com/science/article/pii/S0955286320304368

Midnattsol · Jun 13, 2020

0.04% of the diet by weight or energy..? Either way, it's a lot.

cassava7 · Jun 13, 2020

Midnattsol said: ↑

0.04% of the diet by weight or energy..? Either way, it's a lot.
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By weight, from the study:

After a week, the sham and CKD mice received either a control diet of normal mouse chow (AIN-76A Rodent Diet; GDMLAC) or an identical diet containing 0.04% (w/w) curcumin (purity>98%, USP grade; equivalent dose of 100 mg·kg−1·d−1) purchased from Tianbao Biotechnology Co., Ltd. (Xi'an, Shanxi, China) for 12 weeks of treatment.
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With this huge quantity of curcuma, the improvements in most mito-related measurements between mice w/ CKD and CKD+Cur were in the range of 20-50% (or a little more in Figure 2).

Admittedly though since @MicrobiomDigest and PubPeer have torn down papers from multiple Chinese teams, I am a little skeptical of this particular study.

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Curcumin ameliorates CKD-induced mitochondrial dysfunction and oxidative stress through inhibiting GSK-3β activity, 2020, Wang et al

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Curcumin ameliorates CKD-induced mitochondrial dysfunction and oxidative stress through inhibiting GSK-3β activity, 2020, Wang et al

Andy Committee Member

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