Oral vancomycin treatment suppresses gut trypsin activity and preserves intestinal barrier function during EAE, 2023, Paola Bianchimano et al

Highlights

• Increased intestinal epithelium PAR2 expression and gut permeability during EAE
• Decreased trypsin activity and intact gut permeability in EAE mice on vancomycin
• Gut trypsin activity is modulated by the gut microbiota
• Gut trypsin activity and EAE severity negatively correlate with Lactobacillus taxa

Summary
Studies have reported increased intestinal permeability in multiple sclerosis (MS) patients and its mouse model experimental autoimmune encephalomyelitis (EAE). However, the mechanisms driving increased intestinal permeability that in turn exacerbate neuroinflammation during EAE remain unclear.

Here we showed that vancomycin preserved the integrity of the intestinal barrier, while also suppressing gut trypsin activity, enhancing the relative abundance of specific Lactobacilli and ameliorating disease during EAE. Furthermore, Lactobacilli enriched in the gut of vancomycin-treated EAE mice at day 3 post immunization negatively correlated with gut trypsin activity and EAE severity. In untreated EAE mice, we observed increased intestinal permeability and increased intestinal protease activated receptor 2 (PAR2) expression at day 3 post immunization. Prior studies have shown that trypsin increases intestinal permeability by activating PAR2.

Our results suggest that the interaction between intestinal PAR2 and trypsin may be a key modulator of intestinal permeability and disease severity during EAE.

https://www.cell.com/iscience/fulltext/S2589-0042(23)02220-4?_returnURL=https://linkinghub.elsevier.com/retrieve/pii/S2589004223022204?showall=true

 

Interesting.

I have noted for decades that with increased GI problems, my brain fog, and other cognitive issues worsen. When GI issues are somewhat more under control, my brain fog and other cognitive problems temporarily improve.

However, I find it's not possible to have a satisfactory level of control over GI flare ups. At best, they are only very partially preventable.

 

This would explain the malabsorption issues I had for so many years at the start of M.E. Everything was low, proteins , omega 3, iron et despite eating a very healthy diet and drinking veggie juices ad nauseam.