forestglip
Senior Member (Voting Rights)
Changes in blood metabolic profile during repeated cardiopulmonary exercise testing (2xCPET) in ME/CFS patients
Henrique Chapola, Katarina Lien,, Sissel Dyrstad, Per Ole Iversen, Karl Johan Tronstad
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ME/CFS is characterized by post-exertional malaise (PEM) and delayed recovery after physical activities, possibly indicating a bioenergetic imbalance.
This study analyzed 70 biochemical markers in blood serum, including amino acid metabolites, during repeated cardiopulmonary cycling exercise tests over two consecutive days (2xCPET). Eighteen ME/CFS patients were compared with 15 healthy controls. Samples were taken at rest, peak exercise intensity, and 15 and 60 minutes post-exercise [1].
Multiple tests evaluated significant alterations in concentration and proportional changes. Metabolite enrichment pathway analysis assessed changes in three sets: concentration-related, change between groups, and temporal dynamic behavior. The study explored exercise-induced differences in nicotinamide, tryptophan, BCAA, one-carbon, and butanoate metabolism.
Findings provide insights into ME/CFS physiological response to exercise and biochemical elements of exercise intolerance and PEM. Metabolites overlapping the three sets were further investigated for potential discriminatory value, and their correlation with blood gas parameters was assessed to understand mechanism shifts in ME patients.
Link (Poster)
Henrique Chapola, Katarina Lien,, Sissel Dyrstad, Per Ole Iversen, Karl Johan Tronstad
[Line breaks added]
ME/CFS is characterized by post-exertional malaise (PEM) and delayed recovery after physical activities, possibly indicating a bioenergetic imbalance.
This study analyzed 70 biochemical markers in blood serum, including amino acid metabolites, during repeated cardiopulmonary cycling exercise tests over two consecutive days (2xCPET). Eighteen ME/CFS patients were compared with 15 healthy controls. Samples were taken at rest, peak exercise intensity, and 15 and 60 minutes post-exercise [1].
Multiple tests evaluated significant alterations in concentration and proportional changes. Metabolite enrichment pathway analysis assessed changes in three sets: concentration-related, change between groups, and temporal dynamic behavior. The study explored exercise-induced differences in nicotinamide, tryptophan, BCAA, one-carbon, and butanoate metabolism.
Findings provide insights into ME/CFS physiological response to exercise and biochemical elements of exercise intolerance and PEM. Metabolites overlapping the three sets were further investigated for potential discriminatory value, and their correlation with blood gas parameters was assessed to understand mechanism shifts in ME patients.
Link (Poster)
