Preprint: Specific induction of double negative B cells during protective and pathogenic immune responses, 2020, Ruschil et al

Andy · Sep 12, 2020

Double negative (DN) (CD19+CD20lowCD27-IgD-) B cells are expanded in patients with autoimmune and infectious diseases; however their role in the humoral immune response remains unclear. Using systematic flow cytometric analyses of peripheral blood B cell subsets, we observed an inflated DN B cell population in patients with variety of active inflammatory conditions: myasthenia gravis, Guillain-Barre syndrome, neuromyelitis optica spectrum disorder, meningitis/encephalitis, and rheumatic disorders. Furthermore, we were able to induce DN B cells in healthy subjects following vaccination against influenza and tick borne encephalitis virus. Transcriptome analysis revealed a gene expression profile in DN B cells that clustered with naive B cells, memory B cells, and plasmablasts. Immunoglobulin VH transcriptome sequencing and analysis of recombinant antibodies revealed clonal expansion of DN B cells, that were targeted against the vaccine antigen. Our study suggests that DN B cells are expanded in multiple inflammatory neurologic diseases and represent an inducible B cell population that responds to antigenic stimulation, possibly through an extra-follicular maturation pathway
Click to expand...

https://www.biorxiv.org/content/10.1101/2020.09.08.285148v1?ct=

Snow Leopard · Sep 14, 2020

Ruschil et al. said:

However, DN B cells appear to be a heterogenic B cell subset (30) and have been suggested to represent either exhausted memory B cells / senescent B cells (31), transient effector B cells or a unique atypical memory-like B cells, which may be relevant for plasmablast generation (32,33). More recently, detailed studies in SLE indicated that a subset of DN B cells might be derived from an activated naïve B cell subset and further differentiate into plasmablasts through an extra-follicular maturation pathway (1).
Click to expand...

Low CD20 means Rituximab will have limited clinical response if these cells are pathogenic.

Notably, they did not find an association of increased DN B cells with age, unlike other studies.

Log in or Sign up

Preprint: Specific induction of double negative B cells during protective and pathogenic immune responses, 2020, Ruschil et al

Andy Committee Member

Snow Leopard Senior Member (Voting Rights)

Share This Page

Log in or Sign up

Preprint: Specific induction of double negative B cells during protective and pathogenic immune responses, 2020, Ruschil et al

Andy Committee Member

Snow Leopard Senior Member (Voting Rights)

Share This Page

Useful Searches