Restoration of c-Src/Fyn proteins rescues mitochondrial dysfunction in Huntington’s Disease, 2022, Fão et al

Abstract

Aims: Huntington’s Disease (HD) is an autosomal-dominant neurodegenerative disorder with no effective therapies. Mutant huntingtin (mHTT), the main HD proteinaceous hallmark, has been linked to reactive oxygen species (ROS) formation and mitochondrial dysfunction, among other pathological mechanisms. Importantly, Src-related kinases, c-Src and Fyn, are activated by ROS and regulate mitochondrial activity. However, c-Src/Fyn involvement in HD is largely unexplored. Thus, in this study we aimed to explore changes in Src/Fyn proteins in HD models and their role in defining altered mitochondrial function and dynamics and redox regulation.

Results: We show, for the first time, that c-Src/Fyn phosphorylation/activation and proteins levels are decreased in several human and mouse HD models mainly due to autophagy degradation, concomitantly with mHtt-expressing cells showing enhanced TFEB-mediated autophagy induction and autophagy flux. c-Src/Fyn co-localization with mitochondria is also reduced. Importantly, expression of constitutive active c-Src/Fyn to restore active SKF levels improves mitochondrial morphology and function, namely through improved mitochondrial transmembrane potential, mitochondrial basal respiration and ATP production, but did not affect mitophagy. Additionally, constitutive active c-Src/Fyn expression diminishes the levels of reactive species in cells expressing mHTT.

Innovation: This work supports a relevant role for c-Src/Fyn proteins in controlling mitochondrial function and redox regulation in HD, revealing a potential HD therapeutic target. Conclusion: c-Src/Fyn restoration in HD improves mitochondrial morphology and function, precluding the rise in oxidant species and cell death.

Paywall, https://www.liebertpub.com/doi/10.1089/ars.2022.0001