Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection, 2019, Wohlleber et al

Andy · Jun 13, 2019

Abstract
Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.
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Open access at https://www.nature.com/articles/s41598-019-44922-9

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Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection, 2019, Wohlleber et al

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Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection, 2019, Wohlleber et al

Andy Committee Member

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