Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis, 2025, Gamer, Klimas et al.

Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis
Gamer, Jackson; Van Booven, Derek; Zarnowski, Oskar; Perez, Melanie; Frank, Joshua; Pangeni, Rajendra P.; Collado, Fanny; Klimas, Nancy G.; Oltra, Elisa; Nathanson, Lubov

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic, complex multi-organ illness characterized by unexplained debilitating fatigue and post-exertional malaise (PEM).

We evaluated transcriptomic changes in peripheral blood mononuclear cells (PBMC) of ME/CFS patients undergoing an exercise challenge and explored the transcriptomic response to exercise and recovery in PBMC of ME/CFS patients, as compared to healthy controls using RNA sequencing technology. As transcriptomic changes in ME/CFS patients are still in the phase of discovery, analysis of data has to be stringent, and the most important results have to be validated by a different technology, such as real-time PCR or NanoString.

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