Untargeted metabolomics analysis of rat hippocampus subjected to sleep fragmentation, 2019, Shin et al

Andy · Aug 17, 2019

Highlights

• Several metabolites and specific pathway are altered in the hippocampus by SF.
• The metabolite profiles vary according to the duration of SF.
• The alanine, aspartate, and glutamate metabolism pathway is the most altered pathway.

Abstract
Sleep fragmentation (SF) commonly occurs in several pathologic conditions and is especially associated with impairments of hippocampus-dependent neurocognitive functions. Although the effects of SF on hippocampus in terms of protein or gene levels were examined in several studies, the impact of SF at the metabolite level has not been investigated. Thus, in this study, the differentially expressed large-scale metabolite profiles of hippocampus in a rat model of SF were investigated using untargeted metabolomics approaches. Forty-eight rats were divided into the following 4 groups: 4-day SF group, 4-day exercise control (EC) group, 15-day SF group, and 15-day EC group (n = 12, each). SF was accomplished by forced exercise using a walking wheel system with 30-s on/90-s off cycles, and EC condition was set at 10-min on/30-min off. The metabolite profiles of rat hippocampi in the SF and EC groups were analyzed using liquid chromatography/mass spectrometry. Multivariate analysis revealed distinctive metabolic profiles and marker signals between the SF and corresponding EC groups. Metabolic changes were significant only in the 15-day SF group. In the 15-day SF group, L-tryptophan, myristoylcarnitine, and palmitoylcarnitine were significantly increased, while adenosine monophosphate, hypoxanthine, L-glutamate, L-aspartate, L-methionine, and glycerophosphocholine were decreased compared to the EC group. The alanine, aspartate, and glutamate metabolism pathway was observed as the common key pathway in the 15-day SF groups. The results from this untargeted metabolomics study provide a perspective on metabolic impact of SF on the hippocampus.
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Paywall, https://www.sciencedirect.com/science/article/abs/pii/S0361923019300085
Sci hub, not available

Mithriel · Aug 18, 2019

We now know that sleep is highly dynamic and highly conserved by evolution so it would not be surprising that disruption of sleep has a profound effect on biochemistry. I expect that many diseases and conditions will turn out to have sleep problems behind them

Unfortunately we still don't have ways to initiate the dynamic aspects of sleep artificially; all the present drugs are just sedating.

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Untargeted metabolomics analysis of rat hippocampus subjected to sleep fragmentation, 2019, Shin et al

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Untargeted metabolomics analysis of rat hippocampus subjected to sleep fragmentation, 2019, Shin et al

Andy Committee Member

Mithriel Senior Member (Voting Rights)

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