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two month update: feel quite a bit better. Absolutely unable to confidently tell you if it's the shark liver oil. Lag is a real issue with identifying cause and effect because (1) lag makes it hard to avoid confounders creeping in while you wait for the effect to arrive, and (2) makes doing a...

Interesting!! One of the non-canonical causes of UPR (alluded to above) is a failure of calcium homeostasis in the endoplasmic reticulum. https://cellandbioscience.biomedcentral.com/articles/10.1186/s13578-023-01062-y

Yes, hi ! Not currently but in my most mild periods this is how my mecfs used to remind me it was still lurking. I'd come home from a bike ride all sweaty and full of endorphins, carry on with my day like normal, go to bed, wake up and 24 hours later realise, oh no... The longer i've been sick...

This is such a weird quirk of the disease. If I cross some invisible threshold I get slammed later. It's a massive clue. I previously knew of only one bodily system with a delay built in - the development of antibodies to a novel pathogen. But recently I became aware of another - the end...

I'm always pleased when I see Whitney Dafoe posting online because it suggests he's feeling a bit better. His blog is chugging along recently and he keeps popping up on my instagram. He does seem to have had a big improvement then setback at some point recently because he registered a small...

:emoji_tea::emoji_tea::emoji_tea::emoji_tea::emoji_tea:

I just want to point out that these kind of -omics studies are still coming! Bergquist and Armstrong are going to drop a really detailed study soon, with LOADS of datapoints taken at 20 minute intervals and a couple of thousand metabolites and lipids. I'm keen to wrestle the existing data into a...

Another interesting slide from the same talk, 1h24 mark. Seems to show that a rise in succinate during exercise correlates with mild mecfs. germain Hanson et al 2022 found that patient succinate levels rose less than controls, on average. However they were equivalent before exercise...

INteresting slide from video 2. Via an American researcher called Singh who works in concert with David Systrom. They made two subgroups out of their long covid patients: those who delivered oxygen well and those who didn't. They were able to find the ones who did well had some compensatory...

There are some great points here about the limits of knowledge. I want to describe how I see it. We know most studies cover only a fraction of the metabolites in the body. Even when thousands are measured it's possible there's a smoking gun that we haven't measured or that science can't yet...

Here's another one while I'm on a roll. Naviaux vs Fluge 2021. This is the Fluge paper where they run some unsupervised machine learning to create subsets. This chart has all the subsets bundled together but the next step might be to see how it looks if you plot each separately. code for...

This next chart compares Hanson 2020 to Naviaux 2017. There's not loads of metabolites in common but the ones I found in common do not show strong agreement. Note this chart has lipids and other molecules all mixed in. here's the code for this one...

1. This is so wonderful to see, thanks heaps for your hard work! my dream with this project was the many impressive people in this community would pitch in and it is starting already! 2. Is your topline takeaway from this that hanson 2020 and 2022 have no standout areas of agreement? Is that a...

Great work @chillier ! :heart: i want to come back and respond to that but first I will post the data and code for the analysis I did above. So here's a link for the Hanson 2020 data (clicking this will download a zip file to your computer) https://www.mdpi.com/2218-1989/10/1/34/s1 and here's...

Getting organised to submit an FOI request is very much not my skillset but trawling through 1000 pages of correspondence looking for a smoking gun is my happy place. (Executive function vs hyperfocus; I never had adhd symptoms pre mecfs but i have them now!)

Excellent diligence, Lone wolf! I respect your grind a lot.

This is an excellent question. We can cut it by significance and we should. I'll try to do it myself at some point soon but if someone else wants to have a go before I get to that, that would be delightful! My intial sense is that for some pairs of studies cutting down to metabolites that are...

One possible thing we may be able to point out is that untargeted metabolomics delivers no consistency in findings and isn't worth pursuing. In fact noticing that lit a fire under my curiosity. I'm pretty shocked at how little correspondence I find between different metabolomic studies. For...

I'd like to shout out to a couple of inspirational projects for this kind of meta-analysis. Brydges, Che Lipkin &Fiehn 2023 and Kaczmarek 2023. The former does a Bayesian analysis on 3 metabolomics papers and finds peroxisomes and prostaglandins stand out as important. The latter does a...

So this is some code for analysing the Hanson 2022 data. https://github.com/jasemurphy/mecfs/blob/main/Hanson%202022.R and this is the data (via google drive, you'd need to download it to excel and then load it into R to use the code above.)