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On the topic of whether it is normal or good/bad to have autophagy proteins in serum, the paper says: There's no mention of ATG13 there. For ATG5, a study is referenced that found higher levels in Alzheimers, as well as a study that found lower levels of the same protein, also in Alzheimers...

I'm not on twitter, please do follow up. Yes, it feels a bit like they give us a blow by blow account of everything they tried, and the detailed disease history of the two initial cases who aren't actually included in the larger sample. Which all gets confusing. It might have been better if...

(please correct anything I get wrong) Finding 2: increased autophagy proteins in ME/CFS serum (section 3.3) They used a commercially available autophagy antibody array (i.e. an Elisa method where you have antibodies that bind to the target protein). They started with 1 case control pair, and...

Thanks to those who offered to share the paper with me. There are a lot of potentially interesting findings in this paper. Finding 1: Strong tendency for protein aggregation in the serum of ME/CFS (compared to controls) (Section 3.2) This was done using a thioflavin T assay. On the face of...

It's annoying not being able to see the paper. But the preprint linked in the first post of this now-merged thread gives some insights. On the negative values, there's this: So, I imagine the x axis is increasing concentrations of the serum, and the y axis is the concentration of the target...

Not a waste of effort as far as ginger-indirect moxibustion practitioners are concerned.

Donated. I can't afford much right now, but hopefully another supporter added to the total number will be encouraging.

Donated, I wish the amount could have been bigger. @dave30th, that's quite the photo on your promotional material. I feel sure that you could secure grants and travel opportunities, now that ME/CFS has the much more news-worthy Long Covid angle, and given that you were working on the topic...

from Dynamic association of the ULK1 complex with omegasomes during autophagy induction (formatting mine) I still can't find out how atg13 gets from cells into the blood. I am aware that I'm getting a bit carried away on the basis of barely understood snippets of a preliminary paper. The...

As @Slamdancin suggested above, there's the fact that viral infections cause ATG13 expression to be increased. See for example: Antiviral responses of ATG13 to the infection of peste des petits ruminants virus through activation of interferon response, 2020, Ma et al So, an alternative story...

(I'm working with the snippets only.) So, they tested for 20 autophagy-related proteins in two patients and two controls, and presumably found just ATG13 was different. They then validated the result of elevated ATG13 with 10 or so more patients and controls. I'm assuming the story they are...

This interview is a bit relevant - there is a transcript. Norman Swan talking to Professor Luigi Fontana about research on extending human lifespan. Professor Fontana is keen on calorie restriction. https://www.abc.net.au/radionational/programs/healthreport/the-lowdown-on-longevity/13850872...

Nice idea @Wyva Warm greetings to people with ME/CFS and their supporters in Hungary. There is no credible evidence that it's possible to cure ME/CFS by thinking better thoughts, being more positive or exercising. Anyone suggesting otherwise has not kept up with the scientific literature. Kia...

Stand by for a bigger more expensive trial: These are the organisations that funded the study - clearly they need some advice on trial design.

Ha, it seems people with Long Covid are regarded as too befuddled to contribute to the study design. Patient and public involvement The OHP was tailored to long COVID with input from the long COVID clinic clinicians (two occupational therapists). In addition, guidance was provided on the mode...

Hiyam Al-Jabr 1 Karen Windle 1 David R Thompson 2 Zoe Jenkins 3 David J Castle 4 Chantal Ski 1 1 Integrated Care Academy University of Suffolk Ipswich GB 2 School of Nursing and Midwifery Queen’s University Belfast Belfast GB 3 Mental Health Service St Vincent's Hospital Melbourne AU 4...

Interpreting Magnesium Status to Enhance Clinical Care – Key Indicators, 2017 That paper is interesting, especially if you were planning an Mg supplementation study. There's a good analysis of various ways of determining Mg status (deficiency or otherwise), and also of the time taken for...

While it's true that something labelled 'food grade' is likely to be purer than something that isn't, it seems it isn't the contaminants that is causing the stinging. The linked retailer of the food grade Mg chloride notes 'avoid sensitive skin areas to reduce stinging sensation'. That...

Yes, a mild burning feeling. So, apparently you can make your own magnesium oil from a cup of magnesium chloride crystal dissolved in a cup of distilled water. That's pretty concentrated. But, there are Safety Data Sheets for Magnesium chloride solutions. When it's not packaged up with...

About the burning sensation, I've had that too. I bought a bottle of magnesium oil and tried it - and then did some research and became disillusioned and sprinkled the rest round my citrus trees. Just like the lack of evidence for MgCl solutions being absorbed, I haven't yet found a good...