Ron Davis latest: more evidence of "something in the blood" (Simon M blog)

[alex3619]

well there goes SS31... https://www.biospace.com/article/stealth-bio-s-elamipretide-flunks-mitochondrial-myopathy-trial/

Actually this does not rule out ss31 for ME. In fact it is mildly encouraging as side effects were minimal. It will have to be evaluated separately for ME.

 

[Jaybee00]

Sh*t—well it is still under consideration for macular degeneration—phase 2.

 

[Badpack]

But there goes my plan to try it out in 2020. Because it wont see any admission anytime soon now. :/

 

[Sly Saint]

A simple hypothetical explanation for the cellular response to the salt challenge (in the nanoneedle study) is inhibited Na+/K+-ATPase activity (which is in turn upregulated by cAMP activity). Suggesting altered G protein coupled receptor activity, downregulation of Gs activity or upregulated Gi activity.

I'm not going to even pretend to understand any of this but just wondered if using salt as a stressor could not be used in other testing in lieu of exercise (ie CPET or similar)?

 

[Snow Leopard]

I'm not going to even pretend to understand any of this but just wondered if using salt as a stressor could not be used in other testing in lieu of exercise (ie CPET or similar)?

It's impossible to say until we understand the mechanisms.

 

[Saz94]

Tweet updates from Bhupesh Prusty: https://s4me.info/threads/tweets-from-bhupesh-prusty.13416/

 

[sebaaa]

Merged thread

Outsourcing the 'Something in the blood'

In light of the recent observations from Ron Davis and Dr. Prusty about "something in the blood," which causes excess mitochondrial fission, I was thinking if there's a way to outsource the work of isolating this substance. According to Prusty, he's already found one of them and there's apparently many more varying from patient to patient. It's also the size of an exosome.

Apparently nandixon from the PR forums knew how to do this since she worked as a medicinal chemist, and she also had a hypothesis on what the "something in the blood" could be. I tried to contact her about it, but she hasn't been on the forums since last October now. Here's the quote:

They're not extremely difficult to isolate [EVs]. Note that there's no need to have a pure exosome isolate initially. A crude isolate (e.g., from ultracentrifugation) is good enough for the initial purposes of (1) simply seeing if a generic isolation procedure for exosomes yields a positive result for the "something in the blood" in the nanoneedle - they should have this information already(!), and (2) fractionating the crude exosome contents using liquid chromatography methods to see if the "something in the blood" happens to be in the cargo of the exosome.

. . . I think I understand the challenge pretty well. I probably spent at least 20% of my time as a research medicinal chemist using very similar or identical chromatographic techniques to what they'll need to use.

It may be best just to farm the isolation out to some other group that has experience doing that. (The pdf file that I attached earlier for the especially fast and simple exosome isolation procedure was from a group who was doing the same thing for sarcoidosis.)

Does anyone, especially someone from a research background, know if it's possible to outsource finding the "something in the blood" to researchers that have experience in isolating natural substances or maybe an undergraduate, and do it cheaply? Is this type of commission work possible at all? Are there independent labs that take this kind of commission work assuming that they have have the right equipment?

Source: Here