I hope that you won't mind me commenting on your results. The first screenshot suggests that Amatica is reporting that your mast cell results are unusual. But, your summary of gene expression only has a single supposedly mast cell-related gene where the expression is barely outside the 95% confidence range. I don't know how many mast cell related genes they tested, but it's hard to see how they decided you have a mast cell issue.
Did you understand the point that if you are sampling thousands of genes supposedly related to these areas of interest, everyone will have something that looks a bit odd. Maybe there is some valid information here, but you need to see what the controls' data looks like n order to work out which bit might be useful. It is likely that each control individual also has some gene expression that is unusual. There could be lots of reasons for the variations, and plenty of them aren't related to ME/CFS pathology, especially with such a low number of controls.
Did you understand the point that if you are sampling thousands of genes supposedly related to these areas of interest, everyone will have something that looks a bit odd. Maybe there is some valid information here, but you need to see what the controls' data looks like n order to work out which bit might be useful. It is likely that each control individual also has some gene expression that is unusual. There could be lots of reasons for the variations, and plenty of them aren't related to ME/CFS pathology, especially with such a low number of controls.