Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is a multisystem disease, the pathogenesis of which remains undetermined. We set out to determine the precise abnormalities of gene expression in the blood of patients with CFS/ME. We analyzed gene expression in peripheral blood from 25 patients with CFS/ME diag- nosed according to the Centers for Disease Control and Prevention diagnostic criteria and 50 healthy blood donors, using a microarray with a cutoff fold difference of expression of 2.5. Genes showing differential expres- sion were further analyzed in 55 patients with CFS/ME and 75 healthy blood donors, using quantitative polymer- ase chain reaction.
Differential expression was confirmed for 88 genes; 85 were upregulated, and 3 were downreg- ulated. Highly represented functions were hematological disease and function, immunological disease and function, cancer, cell death, immune response, and infection. Clustering of quantitative polymerase chain reac- tion data from patients with CFS/ME revealed 7 subtypes with distinct differences in Medical Outcomes Survey Short Form-36 scores, clinical phenotypes, and severity.
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.1015.3216&rep=rep1&type=pdf
Open access
Differential expression was confirmed for 88 genes; 85 were upregulated, and 3 were downreg- ulated. Highly represented functions were hematological disease and function, immunological disease and function, cancer, cell death, immune response, and infection. Clustering of quantitative polymerase chain reac- tion data from patients with CFS/ME revealed 7 subtypes with distinct differences in Medical Outcomes Survey Short Form-36 scores, clinical phenotypes, and severity.
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.1015.3216&rep=rep1&type=pdf
Open access