Decreased functional connectivity in post-COVID syndrome patients with high neuroinflammatory activity
INTRODUCTION
Previous research suggests some post-COVID patients with neurocognitive complaints (NCC) show neuroinflammation. Like in inflammatory diseases, this may affect network connectivity. This study aimed to compare resting-state functional connectivity in individuals with and without i) neuroinflammation and ii) persistent post-COVID NCC.
METHODS
Forty-five participants (mean age 49±9 years, 60% female) who had a SARS-CoV-2 infection 27±9 months earlier completed neurocognitive assessment (Checklist-Individual-Strength), 60-minute dynamic [18F]DPA-714 PET scan with arterial sampling for neuroinflammation, and 3T MRI scan for resting-state functional connectivity. Twenty resting-state networks (RSNs) were identified using independent component analysis. Group differences in within-RSN connectivity were analyzed using general linear models. Differences in subcortico-cortical between-RSN connectivity—between brainstem or thalamus and cortical RSNs—were assessed using interaction models.
RESULTS
Ten participants (22%) showed neuroinflammation on [18F]DPA-714 PET, and 31 (69%) reported persistent NCC. Lower within-RSN connectivity was observed in the visual-peripheral (Nvoxels=3151, PFWE<0.05, tmean=1.77 and tmax=3.34) and dorsal-attention networks (Nvoxels=29, PFWE<0.05, tmean=1.59 and tmax=3.66) in those with neuroinflammation, and in the visual-peripheral (Nvoxels=721, PFWE<0.05, tmean=1.89 and tmax=3.45) and default mode networks (Nvoxels=123, PFWE<0.05, tmean=2.24 and tmax=4.68) in those with NCC. A significant interaction effect showed reduced functional connectivity in a large, bilateral cerebellar cluster (Nvoxels=2648, PFWE<0.05, tmean=2.31 and tmax=3.84) with increasing global [18F]DPA-714 binding in participants with NCC. Lastly, thalamic-somatomotor and brainstem-control network connectivity (between-RSN) was altered in both individuals with persistent NCC and those with neuroinflammation, with thalamic-somatomotor changes mainly driven by NCC and brainstem-control changes by neuroinflammation.
CONCLUSION
Our results suggest that neuroinflammation in individuals with persistent NCC after SARS-CoV-2 infection is linked to altered functional connectivity in RSNs central to higher-order cognitive functions.
HIGHLIGHTS
• Lower within- and between-network connectivity on MRI in post-COVID individuals
• Lower connectivity in patients with high neuroinflammatory activity on PET
• Lower connectivity in individuals with post-COVID neurocognitive complaints
• Lower thalamic-somatomotor connectivity is driven by neurocognitive complaints
• Lower brainstem-control connectivity is driven by neuroinflammatory activity
Web | DOI | NeuroImage | Open Access
Denise Visser; Leo Pieperhoff; Luigi Lorenzini; Sandeep Sv Golla; Sander Cj Verfaillie; Anouk Verveen; Elsmarieke van de Giessen; Marijke E den Hollander; Janneke Horn; Caroline M van Heugten; Menno D de Jong; Cees C van den Wijngaard; Tessa van der Maaden; Maria Prins; Patrick Schober; Robert C Schuit; Michael Kassiou; Albert D Windhorst; Brent Appelman; Michele van Vugt; Bart Nm van Berckel; Hans Knoop; Alle Meije Wink; Ronald Boellaard; Frederik Barkhof; Nelleke Tolboom
INTRODUCTION
Previous research suggests some post-COVID patients with neurocognitive complaints (NCC) show neuroinflammation. Like in inflammatory diseases, this may affect network connectivity. This study aimed to compare resting-state functional connectivity in individuals with and without i) neuroinflammation and ii) persistent post-COVID NCC.
METHODS
Forty-five participants (mean age 49±9 years, 60% female) who had a SARS-CoV-2 infection 27±9 months earlier completed neurocognitive assessment (Checklist-Individual-Strength), 60-minute dynamic [18F]DPA-714 PET scan with arterial sampling for neuroinflammation, and 3T MRI scan for resting-state functional connectivity. Twenty resting-state networks (RSNs) were identified using independent component analysis. Group differences in within-RSN connectivity were analyzed using general linear models. Differences in subcortico-cortical between-RSN connectivity—between brainstem or thalamus and cortical RSNs—were assessed using interaction models.
RESULTS
Ten participants (22%) showed neuroinflammation on [18F]DPA-714 PET, and 31 (69%) reported persistent NCC. Lower within-RSN connectivity was observed in the visual-peripheral (Nvoxels=3151, PFWE<0.05, tmean=1.77 and tmax=3.34) and dorsal-attention networks (Nvoxels=29, PFWE<0.05, tmean=1.59 and tmax=3.66) in those with neuroinflammation, and in the visual-peripheral (Nvoxels=721, PFWE<0.05, tmean=1.89 and tmax=3.45) and default mode networks (Nvoxels=123, PFWE<0.05, tmean=2.24 and tmax=4.68) in those with NCC. A significant interaction effect showed reduced functional connectivity in a large, bilateral cerebellar cluster (Nvoxels=2648, PFWE<0.05, tmean=2.31 and tmax=3.84) with increasing global [18F]DPA-714 binding in participants with NCC. Lastly, thalamic-somatomotor and brainstem-control network connectivity (between-RSN) was altered in both individuals with persistent NCC and those with neuroinflammation, with thalamic-somatomotor changes mainly driven by NCC and brainstem-control changes by neuroinflammation.
CONCLUSION
Our results suggest that neuroinflammation in individuals with persistent NCC after SARS-CoV-2 infection is linked to altered functional connectivity in RSNs central to higher-order cognitive functions.
HIGHLIGHTS
• Lower within- and between-network connectivity on MRI in post-COVID individuals
• Lower connectivity in patients with high neuroinflammatory activity on PET
• Lower connectivity in individuals with post-COVID neurocognitive complaints
• Lower thalamic-somatomotor connectivity is driven by neurocognitive complaints
• Lower brainstem-control connectivity is driven by neuroinflammatory activity
Web | DOI | NeuroImage | Open Access
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