Preprint miR-29a-3p, a new myokine orchestrating resistance exercise via coordinated metabolic responses, 2024, Pinto-Hernandez et al.

miR-29a-3p, a new myokine orchestrating resistance exercise via coordinated metabolic responses
Paola Pinto-Hernandez; Manuel Fernandez-Sanjurjo; Daan Paget; Xurde M Caravia; David Roiz-Valle; Juan Castilla-Silgado; Sergio Diez-Robles; Almudena Coto-Vilcapoma; Pau Gama; Pablo M Garcia-Roves; Carlos Lopez-Otin; Juleen R Zierath; Anna Krook; Benjamin Fernandez-Garcia; Cristina Tomas-Zapico; Eduardo Iglesias-Gutierrez

It remains unclear whether the adaptive response to different exercise models is mediated by EV miRNAs released from skeletal muscle and their functional metabolic role.

We sequenced miRNA-loaded plasma EVs obtained from resting mice after 4-weeks endurance or resistance training. Resistance exercise increased the expression of a 11-miRNA profile grouped into two functional clusters. Using both genetically modified animal models and in vitro approaches, we have identified miR-29a-3p as a novel myokine secreted into the bloodstream as EV cargo by contracting skeletal muscle.

It is a cornerstone in the adaptation to resistance training by mediating the coordinated expression and secretion of other miRNAs and affecting muscle mass development and energy metabolism in muscle and liver.

Taken together, our study suggests a coordinating and determinant role of miR-29a-3p in the response and adaptation to resistance training, possibly due to its role as a myokine through its regulatory role in energy metabolism.


Link | PDF (Preprint: BioRxiv) [Open Access]

 

(In mice).

miR-29a-3p having been noted in —

Profile of circulating microRNAs in myalgic encephalomyelitis and their relation to symptom severity, and disease pathophysiology (2020, Nature Scientific Reports)

Circulating microRNA expression signatures accurately discriminate myalgic encephalomyelitis from fibromyalgia and comorbid conditions (2023, Nature Scientific Reports)

Role of the MicroRNAs in the Pathogenic Mechanism of Painful Symptoms in Long COVID: Systematic Review (2023, International Journal of Molecular Sciences)