Phenotypic characteristics of peripheral immune cells of ME/CFS via transmission electron microscopy: A pilot study, Jahanbani et al, 2022

What else can one say but wow….

 

In reference to the Iwasaki/Putrino/Pretorius et al colab:

https://twitter.com/user/status/1589832905769259011


(CD62P is P-selectin.) See thumbnail image in tweet, also frames G and I in the bottom row from the paper's figure 4. (G is a mag of the arrowhead in F)



ETA: in 4I the core around which the platelet rosette has formed is thought to be dead platelets and other cellular debris.

 

Referenced by A Novel Fluorogenic Probe Reveals Lipid Droplet Dynamics in ME/CFS Fibroblasts (2024, Advanced Sensor Research)

 

I wonder if Raman spectroscopy would be useful here -
"Speaker 4: Single-Cell Raman Technologies for Diagnosis and Investigation 25 of ME/CFS Immune Cells - Jiaboa Xu"
https://event.roseliassociates.com/me-cfs-research-roadmap/recordings/

 

Decided I'd email this to Karl Morten & Dr Jiabao Xu - "Hi Karl, Fereshteh Jahanbani posted a link to this paper* on Facebook; there is a reference to Raman**. Seemed to be a positive response to Jiabao's NIH talk - so hopefully NIH will fund further work on Raman. Regards --

**
"Hence, precisely monitoring the dynamic changes of LDs is of particular importance in biomedical research and for early diagnosis of the associated diseases, which includes not only the localization of LDs but also quantitative information such as number and size of LDs in cells and other physiochemical properties such as viscosity, pH, and polarity of LDs. One of the recent advances in LD imaging is the chemical bond-based stimulated Raman scattering (SRS) imaging.[19-21] SRS microscopy allows for the highly specific detection of vibrations of lipid molecules and does not perturb the samples without the requirement of pre-labeling. However, SRS microscopy has limit accessibility for some laboratories due to the complexity and high cost of the equipment. In addition, there are some interferences from other components in the complex biological system, making it challenging to isolate the signal of interest. --