To my knowledge, this is the first study on metabolites in the csf. I believe it's yet unpublished work. It's not clear how differentiated mannose was from controls, but it would be great to finally have something really out of whack. I have no idea what low mannose could mean.
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USA: Center for Solutions for ME/CFS - news and updates from Columbia University's NIH funded center
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To my knowledge, this is the first study on metabolites in the csf. I believe it's yet unpublished work. It's not clear how differentiated mannose was from controls, but it would be great to finally have something really out of whack. I have no idea what low mannose could mean.
The media folks also thought it would be helpful to put a old unplugged microscope plus 3 volumetric flasks on the desk in front of the speakers to give credibility. Same setup/desk used for the Dr Komaroff talk.
Someone’s trying anyway.
Forbin
Senior Member (Voting Rights)
They'll get the hang of it. Columbia's film school was ranked 5th in the country by the Hollywood Reporter. Maybe the Center for Solutions should try to enlist the help of some of their film students, if they haven't already.
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G
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I can't stomach looking at that subreddit. They have pretty awful attitudes toward pwME
Sly Saint
Senior Member (Voting Rights)
eta: new link
CDC
"Columbia University’s Ian Lipkin, MD, visited the CDC in September 2019 to present a special seminar hosted by the Division of High Consequence Pathogens and Pathology’s Chronic Viral Diseases Branch. The presentation covers his work on discovery of unknown pathogens and parallels between myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) and infectious disease and his current, and future ME/CFS research. Comments on this video are allowed in accordance with our comment policy: http://www.cdc.gov/SocialMedia/Tools/... "
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Hoopoe
Senior Member (Voting Rights)
Recently the Lipkin team completed a proteomics pilot study. Small study, but well matched patients from multiple sites.
They found increase or decrease of particular proteins was associated with ME/CFS.
They think ME/CFS may be a final state reachable through multiple different paths (as for example cancer).
They found immunoglobulins associated wit ME/CFS with good odd ratios (all >3).
Immunoglobulin kapp and heavy and light chains which are present at increased concentrations. Findings are consistent with clonal expansion of B cells to specific immunogens.
They're also doing plasma metabolomics (not going to list all the findings).
One of the findings were plasmalogens, which are found in particular in the brain. Known to be altered in alzheimer's disease, so this may be some marker of CNS dysfunction.
Epigenetics pilot study. He says findings are interesting and one of the most promising data. I didn't quite understand it but there appears to be a particular pattern of methylation. Many methylation and expression changes are related to oxidative metabolism as well as stress.
One of areas of interest that emerges from the findings is iron uptake and homeostasis. Various genes, taken individually are not very different, but if taken together they could have a profound effect.
He speculates that there might be a process occurring that is similar to anemia of chronic disease.
Says 2-5% of patients coming into their clinics have evidence of persistent herpesvirus infections.
Talks about a new proteomics study using a chip that can measure the whole human proteome, and stuff associated with herpesviruses and tick borne diseases.
They haven't found the cause but will continue looking at immune history. High throughput sequencing hasn't found any one infectious agent.
He finishes the talk highlighting that this is "a bona fide illness" and a serious problem for society.
There were questions from the audience.
They found increase or decrease of particular proteins was associated with ME/CFS.
They think ME/CFS may be a final state reachable through multiple different paths (as for example cancer).
They found immunoglobulins associated wit ME/CFS with good odd ratios (all >3).
Immunoglobulin kapp and heavy and light chains which are present at increased concentrations. Findings are consistent with clonal expansion of B cells to specific immunogens.
They're also doing plasma metabolomics (not going to list all the findings).
One of the findings were plasmalogens, which are found in particular in the brain. Known to be altered in alzheimer's disease, so this may be some marker of CNS dysfunction.
Epigenetics pilot study. He says findings are interesting and one of the most promising data. I didn't quite understand it but there appears to be a particular pattern of methylation. Many methylation and expression changes are related to oxidative metabolism as well as stress.
One of areas of interest that emerges from the findings is iron uptake and homeostasis. Various genes, taken individually are not very different, but if taken together they could have a profound effect.
He speculates that there might be a process occurring that is similar to anemia of chronic disease.
Says 2-5% of patients coming into their clinics have evidence of persistent herpesvirus infections.
Talks about a new proteomics study using a chip that can measure the whole human proteome, and stuff associated with herpesviruses and tick borne diseases.
They haven't found the cause but will continue looking at immune history. High throughput sequencing hasn't found any one infectious agent.
He finishes the talk highlighting that this is "a bona fide illness" and a serious problem for society.
There were questions from the audience.
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Hoopoe
Senior Member (Voting Rights)
He also said on the topic of herpesviruses, that some clinics have a lot more of these cases than others.
My thoughts follow:
Presumably due to regional differences and what I think is called referral patterns. Anyway according to the data presented herpes viruses appear to be a problem for some patients, but not for most. He seems interested in the topic and wants to explore it further.
My thoughts follow:
Presumably due to regional differences and what I think is called referral patterns. Anyway according to the data presented herpes viruses appear to be a problem for some patients, but not for most. He seems interested in the topic and wants to explore it further.
Tom Kindlon
Senior Member (Voting Rights)
To facilitate sharing:
wigglethemouse
Senior Member (Voting Rights)
In the Q&A Lipkin made a point to state that the NIH funded research centers only received 15-20% of the funding they were promised. This means they can only do small discovery studies.
Lipkin doubts that the pathogen answer will be found with sequencing as they have already looked quite deep that way. That's why looking at the patient samples in response to pathogens and using methods like the chip are the way to go.
Nice to see good collaboration too. Unumatz at Jackson Laboratories, another NIH funded ME research center, has found a subgroup of patients with a specific immune type. Lipkin will analyse this soubgroup of samples to see what else they have in common.
This chip looks very interesting. It's based on a previous chip that costs $3,500 but they reconfigured it for ME/CFS and reduced the size to lower the cost, but still very expensive. Currently have 40 patient and control samples collected, want to get to 50 before starting the work.
Lipkin doubts that the pathogen answer will be found with sequencing as they have already looked quite deep that way. That's why looking at the patient samples in response to pathogens and using methods like the chip are the way to go.
Nice to see good collaboration too. Unumatz at Jackson Laboratories, another NIH funded ME research center, has found a subgroup of patients with a specific immune type. Lipkin will analyse this soubgroup of samples to see what else they have in common.
Forbin
Senior Member (Voting Rights)
I didn't completely follow this, but he mentioned herpes viruses a couple of times.
Later when talking about an expensive sequencing test he mentions:
Later still, when talking about developing animal models to test, he says:
I could have this wrong, but it sounds like the persistence of herpes viruses may be related to their triggering the methylation (suppression) of certain host genes which might otherwise work to eliminate these viruses. They may be looking to see if this method of persistence might be related in some way to the persistence of ME/CFS. [Just my guess based on what he said.]
Later when talking about an expensive sequencing test he mentions:
Later still, when talking about developing animal models to test, he says:
I could have this wrong, but it sounds like the persistence of herpes viruses may be related to their triggering the methylation (suppression) of certain host genes which might otherwise work to eliminate these viruses. They may be looking to see if this method of persistence might be related in some way to the persistence of ME/CFS. [Just my guess based on what he said.]
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Hip
Senior Member (Voting Rights)
When I asked Prof Lipkin whether his sequencing technique on blood samples would be able to detect enterovirus infections in the tissues, he said no it would not. So for any enteroviruses (and possibly other viruses) infecting the tissues of ME/CFS patients, sequencing is blind to such infections.
EDIT: unless you perform the sequencing on the tissues themselves.
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